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Neuraminidase (exo-α-Sialidase)

from Vibrio cholerae, solution

For further processing only.

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Order information
Neuraminidase (exo-α-Sialidase) material number and pack size:
Material Number Pack Size
11087096103 custom fill
Will be supplied as "Neuraminidase (Sialidase)". Unit of measure is "U".
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Overview
Neuraminidase is a glycohydrolase.
Neuraminidase hydrolyzes terminal N- or O-acyl-neuraminic acids that are α2,3-, α2,6-, or α2,8-linked to galactose, Hex, NAc, or N- or O-acylated neuraminyl residues in oligosaccharides/glycoconjugates or colominic acid. Relative rate of cleavage is α2→3 >α2→6 >α2→8, determined on bonds in tri- and tetrasaccharides.
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Application
Neuraminidase hydrolyzes terminal N- or O-acetylneuraminic acids which are α2,3-, α2,6-, α2,8- linked to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids. For the hydrolysis of glycolipids, the presence of a detergent is necessary. Roche's Neuraminidase is very well suited for structural research studies on glycoconjugates and for hydrolytic cleavage of sialic acid from biological material.
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Specification
Molecular weight: Approximately 95 kD
pH optimum: 5.5-6.2
Specific activity: Approximately 20 U/mg total protein (approximately 40 U/mg enzyme protein at +37°C) and pH 5.5 with N-acetyl-neuraminosyl-D-lactose as the substrate.
Appearance: Clear, colorless solution
Contents (after blending with Micr-O-protect® and EDTA): Natrium acetate, 50 mmol/L; NaCl, 154 mmol/L; CaCl2, 9 mmol/L; EDTA, 10 mmol/L; Micr-O-protect® 0.1% (w/v)
pH value: 5.0-6.0
Activity (+37°C, with N-acetylneuraminyl-lactose): ≥1.0 U/mL
Stability: At +2 to +8°C within specification range for 18 months.
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License disclaimer

78:The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.