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β-1,4-Galactosyltransferase, rec. EQ

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For further processing only.

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Order information
β-1,4-Galactosyltransferase, rec. EQ material number and pack size:
Material Number Pack Size
08098182103 available in 0.2 g per bottle
Unit of measure is "gram".
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Overview
β-1,4-Galactosyltransferase is used for in vitro galactosylation of all GlcNAc1-2Man units on glycoproteins, such as monoclonal antibodies (MABs).
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Application
β-1,4-Galactosyltransferase, rec. EQ is used for targeted, in vitro galactosylation of glycoproteins, which are not, or not fully galactosylated.
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Specification
A generic protocol for galactosylation of glycoproteins is available. However, the optimal conditions depend on different factors (e.g., the initial and target glycosylation status of the  glycoprotein) and must be determined by the user.
Appearance: Clear and colorless to slightly yellowish solution
Composition: 50 mM Tris-HCl, 100 mM NaCl, pH 8,4 at 20°C
Specific activity: >12 U/mg in Galactosyltransferase activity assay
Protein concentration: 15 ± 1.5 mg/mL
Purity (HPLC): ≥90%
Bioburden: ≤100 CFU/mL (in evaluation)
Endotoxin: ≤10 EU/mg (in evaluation)
Host cell protein (ELISA): ≤ 4000 ppm
Host cell DNA (qPCR): ≤ 100 pg/mg
Metals: (Cd, Co, V, Ni, Ag, Se)  ≤ 10 ppm
No animal-derived material is used in fermentation, purification and final formulation of this enzyme
Stability at -15°C to -25°C: within specification range for 12 months.
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License disclaimer

78:The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.