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α-2,6-Sialyltransferase, rec. EQ

human, expressed in CHO cells, solution

For further processing only.

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Order information
α-2,6-Sialyltransferase, rec. EQ material number and pack size:
Material Number Pack Size
08098174103 available in 0.4 g per bottle
Unit of measure is "gram".
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Overview
α-2,6-Sialyltransferase, rec. EQ can be used for in vitro glycoengineering of glycoproteins, e.g. therapeutic proteins, in drug development and manufacturing.
α-2,6-Sialyltansferase, rec. EQ is a highly active recombinant human glycosyltransferase, expressed in CHO cells.
The activity of this enzyme is comparable to the activity of α-2,6-Sialyltansferase, rec.

To use in vitro glycoengineering in drug development and manufacturing processes, enzymes should be of enhanced quality (EQ), compared to enzymes used in early
R&D or analytical applications: Enzymes should be tested for host-cell impurities and relevant metal impurities and be available at least in gram amounts .
α-2,6-Sialyltansferase, rec. EQ fulfills those criteria and can also be provided in GMP Grade upon request.
Benefits of using in vitro glycoengineering to modify glycosylation of therapeutic proteins:
  • Time- and cost-saving generation of glycan variants to develop improved drugs
  • Optimized glycosylation without compromising other CQAs or product yield
  • Improved lot-to-lot consistency to reduce risk of product quality variation and resulting delays
  • Generation of glycoprofiles which may not otherwise be generated
  • Streamlined analytics in comparability studies
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Application
Incubation of α-2,6-Sialyltansferase and CMP-NANA with a glycoprotein leads to increased sialylation of the glycoprotein.

As α-2,6-Sialyltansferase rec. EQ adds sialic acid only to galactosylated glycostructures, a galactosylation of the glycoprotein in a previous step helps to achieve higher sialylation levels. Galactosylation can be achieved by incubation of the target protein with ß-1,4-Galactosyltransferase and UDP-Galactose.
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Specification
Appearance: Clear and colorless to slightly yellowish solution
Specific activity: ≥400 U/µg
Protein (OD 280 nm) A280 = 1.931: 15 ± 1.5 mg/mL
Purity (HPLC): >90%
Bioburden: ≤100 CFU/mL
Endotoxin: ≤10 EU/mg
Host cell protein: ≤ 4000 ppm
Host cell DNA: ≤ 100 pg/mg
Heavy metal (Cd, Co, V, Ni, Ag, Se): ≤ 10 ppm
Stability: At -15 to -25°C within specification range for 12 months.
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License disclaimer

78:The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.