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β-1,4-Galactosyltransferase

solution

For life science research only. Not for use in diagnostic procedures.

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Order information
β-1,4-Galactosyltransferase material number and pack size:
Material Number Pack Size
07215118103 custom fill
Unit of measure is "mg".
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Overview
β-1,4-Galactosyltransferase is used for in vitro galactosylation of all GlcNAc1-2Man units on glycoproteins, such as monoclonal antibodies (MABs).
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Application
β-1,4-Galactosyltransferase is used for targeted, in vitro galactosylation of glycoproteins, which are not, or not fully galactosylated.
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Specification
A generic protocol for galyctosylation of glycoproteins is available. However, the optimal conditions depend on different factors (e.g., the initial and target glycosylation status of the glycoprotein) and must be determined by the user.
Molar mass: 39.5 kDa by cDNA
Appearance: clear, colorless solution
Composition: 50 mM Tris/HCl, 100 mM NaCl, pH 8.4 at +20°C
Volume activity: ≥60 U/mL
Specific activity: ≥12 U/mg
Protein concentration: 5.5 ± 0.5 mg/mL
Purity and identity (SEC-HPLC): ≥90%
Bioburden ≤100 CFU/mL (in evaluation)
Endotoxin ≤10 EU / mg (in evaluation)
No animal-derived material is used in this process.
Stability: At -15°C to -25°C: within specification range for 12 months.
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License disclaimer

78:The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.