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N-Glycosidase F (PNGase F)

N-Glycosidase F of Flavobacterium meningosepticum, recombinant from E. coli, lyophilizate

For further processing only.

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Order information
N-Glycosidase F (PNGase F) material number and pack size:
Material Number Pack Size
06538355103 500 U/vial
Unit of measure is "piece".
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Overview
N-Glycosidase F cleaves asparagine-bound N-glycans from glycoproteins.
N-Glycosidase F is a recombinant, Peptide-N4-(acetyl-β-glucosaminyl)-asparagine amidase from Flavobacterium meningosepticum, expressed in E. coli, provided as a lyophilizate.
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Application
N-Glycosidase F can be used in glycoprotein analysis to cleave asparagine-bound N-glycans from glycoproteins, allowing glycan and protein to be analyzed separately.
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Specification
Appearance: White lyophilized substance
Solution (after dissolution in water;c = bottle / 0.25 U/mL water): Clear, colorless solution.
Activity (at 37°C; with Dabsyl-fibrin-glycopeptide, HPLC): ≥500 U/bottle. ≥50 U/mg lyophilized material.
Specific activity: ≥25 000 U/mg protein
Purity-SDS-PAGE (not reducing): ≥90 area%
Contaminants expressed as a percentage of N-Glycosidase F activity:
Endoglycosidase F: not detectable
β-N-Acetylhexosaminidase: A/17h≤0.025%
α-Fucosidase: A/17h ≤0.025%
β-Galactosidase: A/17h ≤0.025%
β-Glucosidase: A/17h ≤0.025%
α-Mannosidase: A/17h ≤0.025%
β-Mannosidase: A/17h ≤0.025%
Sialidase: A/17h≤0.025%
Proteases (casein-resorufin): A/17h ≤0.025%
Stability: At +2 to +8°C within specification range for 24 months.
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License disclaimer

78:The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.