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N-Glycosidase A (PNGase A)

solution

For further processing only.

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Order information
N-Glycosidase A (PNGase A) material number and pack size:
Material Number Pack Size
11646583103 custom fill
Will be supplied as "N-Glycosidases A, Solution". Unit of measure is "milliU".
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Overview
Enzyme that cleaves N-glycans.
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Application
Use N-Glycosidase A to cleave all types of asparagine bound N-glycans including high mannose-, hybrid-, biantennary-, triantennary- and tetraantennary complex types, provided that the amino group as well as the carboxyl group are present in peptide linkage. Use N-Glycosidase A also to cleave a single N-acetylglucosamine residue from the peptide.
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Specification
Appearance: Clear, colorless solution
Activity: ≥50 mU/mL
Specific activity (Bradford): ≥0.5 U/mg protein
Contaminants (expressed as percentage of N-Glycosidase-A activity):
α-Galactosidase: ≤0.1
β-Galactosidase: ≤0.1
β-Glucosidase: ≤0.1
α-Mannosidase: ≤0.1
β-Mannosidase: ≤0.1
β-N-Acetyl-glucosaminidase: ≤0.1
β-Xylosidase: ≤0.1
α-Fucosidase: ≤0.1
Sialidase (after 17 hours at +37°C): ≤0.1%
Proteases (casein-resorufin, after 17 hours at +37°C): A/17h ≤0.025
Stability: At -15 to -20°C within specification range for 24 months.
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License disclaimer

78:The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.